| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028971 |
| Chromosome: | chromosome 14 |
| Location: | 1244681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616000 | (1 of 35) IPR001763 - Rhodanese-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTCCCGCGCGCAGCCCAGTGGTTTCGTCACGTGTCGGGAGGCGCTGC |
| Internal bar code: | ACGTGTAGGGCTCCTGTGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2917 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAATCCGTGCCCTCAGTC |
| Suggested primer 2: | GATGCGATTCGTGCAGGTTC |