Insertion junction: CLIP2.028977_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028977
Chromosome:chromosome 6
Location:4172377
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278234 FAP343 (1 of 19) PF00397 - WW domain (WW); Flagellar Associated Protein 343 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACACACACGCACACACGCCTCACCTTCTCCAGCTCCGCCTGGCTC
Internal bar code:AGTGATATAACCAGGGACAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1890
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ACCATCTCAACGCCCAGAAG
Suggested primer 2:TCAGGTCCCGTCACCAAAAG