Insertion junction: CLIP2.029000_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029000
Chromosome:chromosome 5
Location:1006850
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247300 (1 of 1) PF13432//PF13489 - Tetratricopeptide repeat (TPR_16) // Methyltransferase domain (Methyltransf_23) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTCACCAGCCGCCACCCCGCCTCGCCGCCCACACGCCGCAGGTACTC
Internal bar code:TGTCATGATGGTGAATCGTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1203
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGACACCATGACCACGACAG
Suggested primer 2:CTGCATGTTGTTGCTGCTGT