Insertion junction: CLIP2.029004_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029004
Chromosome:chromosome 7
Location:207751
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g313500 NUP214 Nucleoporin 214 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGGGTGGGCCGGACCTGTGCGCGCGGCGGCGACGCCGGCTCGCCCGCG
Internal bar code:AAAGCGAGGCGTGTGCTCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:269
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAGATGCATTGGCTGTGACG
Suggested primer 2:CAGCGTCAGATCAGGTCGAA