Insertion junction: CLIP2.029018_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029018
Chromosome:chromosome 2
Location:5775905
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g116850 HLM6,SETb (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Histone-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGATTCCCGGCCGCGGCCGCGGTCTGGTGTCCTGCCGCCCCATCGCCG
Internal bar code:GTATCGGTGTCAAACTAAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1063
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCATCTCCATGAAGGCCA
Suggested primer 2:CATCCACCACCTCAACCTCC