Insertion junction: CLIP2.029024_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029024
Chromosome:chromosome 3
Location:2505659
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g159400 (1 of 2) 2.7.7.73 - Sulfur carrier protein ThiS adenylyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTCAAGCAGATTCTGCCAGAGGTAGGACCGGGGTGGGATATTTCGAG
Internal bar code:TACGCTTGCACTGGCGGCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3311
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGCAACACCCCCTTTT
Suggested primer 2:AAGTGCCTTCGTGGTAGTCG