Insertion junction: CLIP2.029032_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029032
Chromosome:chromosome 10
Location:651303
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421950 TEF26 (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754); Predicted protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTAGTAGACCTTGAACTCCAGGTACTCGTCACTGGCCGAGTGCACGTT
Internal bar code:CCATTTACTTCCGGTTACGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1288
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTGCTACTGTGTCTGTT
Suggested primer 2:TGAAGGTGCAAACCAGGTGT