| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029033 |
| Chromosome: | chromosome 12 |
| Location: | 1149186 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490850 | (1 of 1) PTHR10527//PTHR10527:SF6 - IMPORTIN BETA // IMPORTIN-4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCGAACTCCTTGCCCGCCGCCGCCACCACGGACGCCAGAGCCGACA |
| Internal bar code: | AACCTTTACGTGCCGAAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGATCGTGGAGCACTAC |
| Suggested primer 2: | GGCTGGTAAGTACAACCCCC |