| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029035 |
| Chromosome: | chromosome 12 |
| Location: | 4979875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524900 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | CDS | |
| Cre12.g524950 | ENGB,ENGB1 | Factor possbly involved in ribosomal 50S subunit assembly; (1 of 1) PTHR11649//PTHR11649:SF49 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAATGCATAAGGGAAGCGCAGGCCCATGCAGAAAATTTGCACAGTGTC |
| Internal bar code: | AGTTGTCTTTCAGTAGACTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3133 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCCGAGGAGCCTGATGG |
| Suggested primer 2: | TCACTTGCAGCTCCGTCAAT |