| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.029043 |
| Chromosome: | chromosome 4 |
| Location: | 2055903 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219350 | PLC1 | (1 of 14) IPR017946 - PLC-like phosphodiesterase, TIM beta/alpha-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGTTTGGGCCCCCCAAAGGGCTCAGCTACCACGCCATCCGTTGAGC |
| Internal bar code: | ATTAAGATGTGCTGCTTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGTATTGATGCGAGGGC |
| Suggested primer 2: | AGCTTGCGAGATGCTGTTCT |