| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029046 |
| Chromosome: | chromosome 10 |
| Location: | 6013450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801202 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATGGCCAAGGCAGCTTTCGCAGAAGCTGTGGAGGTGGCAGCAGGCGC |
| Internal bar code: | TCTCTTAAGACGTGTCAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2253 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGGTGGCGATCAGGAAG |
| Suggested primer 2: | ATGTGTGGGATGAGTTGCGT |