Insertion junction: CLIP2.029052_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029052
Chromosome:chromosome 3
Location:1296657
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g150200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACGCC
Internal bar code:CGGGTCTATAGGGGGACGTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGGAAGTGTGGTTCAGA
Suggested primer 2:GACCCAACCCAACCCTGTAG