| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029116 |
| Chromosome: | chromosome 3 |
| Location: | 123115 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143847 | (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCGCTAGTAGTGGCCGAAAGTGCTGCGAGAACATGACATCATTTCC |
| Internal bar code: | ACTACAAAGCATTGTTTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2042 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTCCAGGATAGGATGC |
| Suggested primer 2: | TCTGGAAGGGGGCATACGTA |