| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029135 |
| Chromosome: | chromosome 1 |
| Location: | 2454971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014250 | OPR2 | OctotricoPeptide Repeat protein 2 | 3'UTR |
| Cre01.g800044 | (1 of 39) PTHR13078:SF53 - PROTEIN MAOC-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTTATCTTGGTAGGGCCAACAGCGGACTCGCCACCGGCCTCACCCT |
| Internal bar code: | GTACCGAGGGCGGGTTCTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3414 |
| LEAP-Seq percent confirming: | 87.037 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATGGCTCTGCTTCCCT |
| Suggested primer 2: | GGCAACCCAGCTTCAACTTG |