| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029136 |
| Chromosome: | chromosome 3 |
| Location: | 9114477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200879 | CSR10 | Carbohydrate sulfotransferase-related 10; (1 of 1) IPR000104//IPR000863//IPR012884//IPR027417 - Antifreeze protein, type I // Sulfotransferase domain // Excisionase-like // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCGCGCGTGCCTGGGAACTCAGGGCGCTGCGGCTGGCGCAGCATCC |
| Internal bar code: | ACGTAGCCGCTAGTCTCAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTTGTTGTCGCACAGG |
| Suggested primer 2: | CCGTCTTTCCCTTCGCTTCT |