| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.029137 |
| Chromosome: | chromosome 16 |
| Location: | 5202867 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684700 | (1 of 1) PF10309 - Protein of unknown function (DUF2414) (DUF2414) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCGGGAACAAGACGTTGCCTGCGGAGGGAGGTGAAGGTGTTTTGTGA |
| Internal bar code: | TTGACACTGTAGTTATAACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTCGGAGACTACGGCG |
| Suggested primer 2: | AGCGTCCCTTATCACCTCCT |