| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.029183 |
| Chromosome: | chromosome 16 |
| Location: | 60760 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695851 | (1 of 2) 2.1.1.229 - tRNA (carboxymethyluridine(34)-5-O)-methyltransferase / tRNA methyltransferase 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGCAACCGTGGCAGCAGCGGCGGAGGTAGCAGCCAGAGCAGGGGCG |
| Internal bar code: | TTTCGCGGTACTATCTACAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1763 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTTCCTACACCACCTG |
| Suggested primer 2: | AACTCCTCGAGCCTTGCTTC |