| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.029186 |
| Chromosome: | chromosome 12 |
| Location: | 5260826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527800 | DNAL4,LC10,ODA-LC10,DLC10,DLL3,MOT24 | Outer Arm Dynein Light Chain; (1 of 1) K10412 - dynein light chain 4, axonemal (DNAL4) | CDS |
| Cre12.g527850 | TRA1 | (1 of 1) PTHR10741//PTHR10741:SF5 - TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X // TRANSLIN-ASSOCIATED PROTEIN X; Translin-like protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGAGGTACGAGCTGGAAAAAACGCACCGCCTAGCAAGCAGAGCAGGC |
| Internal bar code: | GGTTACGATCTAGGTAAAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2445 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCAGTGTGGCTGTGTA |
| Suggested primer 2: | TGACGTCTTTTACCAGGCCC |