Insertion junction: CLIP2.029202_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029202
Chromosome:chromosome 12
Location:5062141
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g525550 MPA10 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 2) PTHR12905//PTHR12905:SF0 - SPERM PROTEIN HOMOLOG // METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGTTGCCCGGCGCACCGCCCTTTCAACGGCTAGGTTCCACGACACCA
Internal bar code:CCTATGGGATCGGCGATGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2235
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGTGGCATTTTACCGCGG
Suggested primer 2:GAGGTTAGGAAGCTCGGCTC