Insertion junction: CLIP2.029205_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.029205
Chromosome:chromosome 12
Location:3325683
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g498700 CPLD13 Conserved in the Plant Lineage and Diatoms CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGCAGCGCGTAGATGCCTAGCGAGCGAGGCGGCGGAGTCACCACTTG
Internal bar code:GGTTGATCTGTAATCCCCCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2120
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCACAGTGCTCAAAACC
Suggested primer 2:CGGTTGTGCAGTGAACTGTG