Insertion junction: CLIP2.029206_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029206
Chromosome:chromosome 9
Location:1732401
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g398400 TRP5 Transient receptor potential ion channel protein; (1 of 46) PF00520 - Ion transport protein (Ion_trans) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGAACGCTGAGCGGGCGGCTGGTGCGGTCTAAATCGGCGCGACTGAGG
Internal bar code:ACGCGCACGACCGACTCTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TACACCACCGCCTTAACGTC
Suggested primer 2:GCCAGACAGACATCAACCCA