| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029215 |
| Chromosome: | chromosome 10 |
| Location: | 3362005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443600 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCATGCTGTGGCCTGCATGCCTGTCGGCAGGTGCGCTACGCCTCGCCT |
| Internal bar code: | GGTTTGCTTAAGAGGGGTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2213 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCTCATCGGGCAGTG |
| Suggested primer 2: | AGCGGTGTGTTTGACTCGAT |