Insertion junction: CLIP2.029226_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029226
Chromosome:chromosome 17
Location:1407119
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g706200 (1 of 1) IPR006029//IPR006201 - Neurotransmitter-gated ion-channel transmembrane domain // Neurotransmitter-gated ion-channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAGAACAGGACCGCCTCGGTGGGGTTGCTATTGCCGGCGCCGGCTCC
Internal bar code:TCCTACCCGCTGCAATATTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:861
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCCCTTCGACTGGCAGTC
Suggested primer 2:ACATACCAGTCTAGGCGGGT