Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.029244 |
Chromosome: | chromosome 2 |
Location: | 2969227 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095300 | (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTACCGTGGTTAAGTCGATGGTGTTGATACACGCGCTGTCAAGTCGGC |
Internal bar code: | GTGCGAATTGGGCACTGCATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 669 |
LEAP-Seq percent confirming: | 54.5455 |
LEAP-Seq n confirming: | 30 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAAGCTTTGAGCCCCCATC |
Suggested primer 2: | TGGGGCAGATACGGTAGACA |