| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.029246 |
| Chromosome: | chromosome 9 |
| Location: | 2184233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395450 | (1 of 9) PF01344 - Kelch motif (Kelch_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGATGGCGGCGCCGCCACCCACGTACACCAGCTCAGTCCTGCGGGTG |
| Internal bar code: | TGTGGGATAATCCCGGGTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1356 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAATCATCATGCCACGC |
| Suggested primer 2: | CTACGCGCGTGAGATTGTTG |