| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.029305 |
| Chromosome: | chromosome 2 |
| Location: | 4033845 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103000 | FKM3 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACTCACCTCGCGCAGGGCCGGCAACGGTGCGTCCCAGCCGCCAGAG |
| Internal bar code: | ATGGACGAGATGTTTAAGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2215 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCAAGGACGAGATCGAG |
| Suggested primer 2: | GGGTCCTTGGGGTCTAGGAT |