Insertion junction: CLIP2.029313_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029313
Chromosome:chromosome 3
Location:5962161
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189150 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCATCTGGGTGGGCTTCGTCCTGTATTCTTTGTGCACACACATCCACA
Internal bar code:AATTCGATCCATAACAGCAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGGTCGAAGTTTCCGCT
Suggested primer 2:GAATGGGAGTGGTGGGAGTG