| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029319 |
| Chromosome: | chromosome 12 |
| Location: | 1351140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489200 | (1 of 1) PTHR10794//PTHR10794:SF28 - ABHYDROLASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTACTGCAGACATCGGTGTAAAAGTAGCTACCGGACACTGAGCGCTG |
| Internal bar code: | TAGAGTCCGCGTAATCACTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3588 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGATGGTGGTCGCCAATT |
| Suggested primer 2: | TACCTCCTCCATGTCAGCGA |