| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029330 |
| Chromosome: | chromosome 10 |
| Location: | 3030980 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440750 | TOP3 | Topoisomerase type III, organellar DNA gyrase; (1 of 1) K02470 - DNA gyrase subunit B [EC:5.99.1.3] (gyrB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCAGCGCATCCAGCGACAGGCGGCGGCTGTAGGCCTCAATGAGCGCC |
| Internal bar code: | AGGACGATGAAAAAGGCGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1321 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGATGTCACGCCTTCAA |
| Suggested primer 2: | GAGCATGGGAGCACTTCTGT |