Insertion junction: CLIP2.029344_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029344
Chromosome:chromosome 3
Location:7852524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g202850 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCGAGACCAGAACTACAAGGTCTCAAAACACACCAAGTGAGCGGCGGG
Internal bar code:AGTGGAAAATTCGTTTGGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:5003
LEAP-Seq percent confirming:98.2759
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:GTATTCCGAAGACGCCCCAA
Suggested primer 2:GAGCTGTTTGACTCCGGTGA