Insertion junction: CLIP2.029352_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029352
Chromosome:chromosome 10
Location:2769404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438300 LTH4 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease; LAT2 homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCTGCTACCCTTTTATTATCTCCCTAGGGCTGCCGTTCCGCCCTTTG
Internal bar code:TAGCATTTCGCACCCAGTCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:5053
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:CATCGTGTGGACCACCTTCA
Suggested primer 2:ACGTGCTCCTTTAGGAAGCC