| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.029354 |
| Chromosome: | chromosome 17 |
| Location: | 2368587 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g714100 | HTA21,HTA32 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGGAGCTGGCCGGCAACGCCGCCCGCGACAACAAGAAGAACCGTAT |
| Internal bar code: | TCTGTAGAATTTTGCGTGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2091 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTGCTCAGGATTGGGT |
| Suggested primer 2: | TTATTTGACCCAACCGCCGA |