| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029379 |
| Chromosome: | chromosome 16 |
| Location: | 4032268 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690100 | FAP213 | Coiled-Coil Flagellar Associated Protein 213 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAACCGGTCATACTCGTCGCAGAAGGCTGGGGCATTCCGTGCCGGCAT |
| Internal bar code: | CCCACAAGTACACGAGGAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1872 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCATCGGAGGAACGTC |
| Suggested primer 2: | GTCATCACATGCCCTTTCGC |