| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029388 |
| Chromosome: | chromosome 3 |
| Location: | 5261967 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800345 | (1 of 1) PTHR23304//PTHR23304:SF106 - SPOT2-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTCAGGGTCAGGGTCAGGGTCAGGGTCAGGGTCAGGGTCAGGGTCAG |
| Internal bar code: | AATTTATTTTGACTTGTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4249 |
| LEAP-Seq percent confirming: | 90.6977 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCCTCTAGCTACCCCAC |
| Suggested primer 2: | CCACCAAGTCAGGGTCCATC |