| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029399 |
| Chromosome: | chromosome 12 |
| Location: | 3131874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500715 | (1 of 5) 6.2.1.3 - Long-chain-fatty-acid--CoA ligase / Lignoceroyl-CoA synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGCCAGGTCATCCGGCGCAGGAGGAACCGCCGGCAGCCACGGCGC |
| Internal bar code: | GCCTGTCCTCAGTTGAACGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGGACGAGCTACAG |
| Suggested primer 2: | GATGCCAACAACAACCCACC |