Insertion junction: CLIP2.029462_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.029462
Chromosome:chromosome 8
Location:671913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360400 ERM1 (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTTCCCCCCTCACCGCGCAGCTCGCTGCGGCGTCGCCAACACCACAC
Internal bar code:CGCTTATGGACCAGGAATAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2876
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACTGCGTGACCTGGTTG
Suggested primer 2:ATGGTATTGCCGCTCCAACA