Insertion junction: CLIP2.029470_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.029470
Chromosome:chromosome 11
Location:4007141
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g480451 PHC42 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAACACACAACCCATCCCATCCCGTCCCAGCCCGCTCCCCCCGCAAA
Internal bar code:CCGGTGGTCTGATGACAATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3919
LEAP-Seq percent confirming:98.1481
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:108

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGGATGGCGCTATAACG
Suggested primer 2:TTGACCTCCGTGTACTTGCC