Insertion junction: CLIP2.029477_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029477
Chromosome:chromosome 1
Location:6438230
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g045600 SRR12 (1 of 1) PTHR24363//PTHR24363:SF0 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Speract/scavenger receptor, transmembrane glycoprotein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAATCCCCCGCTGGTGGTGCTGTACATCTCCTCGTGAACCCTCCACCC
Internal bar code:ATGCTTACGACGACTGCAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:573
LEAP-Seq percent confirming:86.3636
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCATCCCTCCTCACTCC
Suggested primer 2:GACTCACAGGGTGGTGAAGG