Insertion junction: CLIP2.029486_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029486
Chromosome:chromosome 8
Location:4196927
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g383800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTCCTCCCTACATCGAGTCCAGCGCTCCAACCGCCTCCAACCGCCTGC
Internal bar code:CAGCATAGGAAAGCTGTCTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1868
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTCCCGTATTGGTCGAC
Suggested primer 2:GAGGACTCGTGTGATAGGCG