| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.029510 |
| Chromosome: | chromosome 2 |
| Location: | 4330373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105050 | (1 of 1) PTHR34810:SF1 - DNA-BINDING PROTEIN BIN4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGGTACAGGTGTTGGCACAGCTGCGCACAGCACGCGCGCACGACTGC |
| Internal bar code: | GGGGATGGTACTGACGATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4512 |
| LEAP-Seq percent confirming: | 87.8378 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAAGTTCCAGTGCACCG |
| Suggested primer 2: | GGAGGAGAGGGAAAGGCCTA |