Insertion junction: CLIP2.029516_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029516
Chromosome:chromosome 10
Location:1540884
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g428750 (1 of 1) K14792 - rRNA biogenesis protein RRP5 (RRP5, PDCD11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCGTGCAAAGCGCTTGAGTGAAGCGTTTGCTGACATGCAGCCAGTGC
Internal bar code:GCCAGGACTTTAGTATATGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:917
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGTTCTTGACAGCAGC
Suggested primer 2:AACCCAGAGATGCAATGCGA