| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.029517 |
| Chromosome: | chromosome 7 |
| Location: | 5666274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352650 | (1 of 2) PTHR23139//PTHR23139:SF58 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGACCGGCATCACCATCCCCGTGCCGCCGCCCACCGTGTCCGACAGG |
| Internal bar code: | GGTGAAAGAGCTAGGGCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGCCTGATACCGTGATA |
| Suggested primer 2: | GAGGGCGAATATGAGGGAGC |