| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029521 |
| Chromosome: | chromosome 10 |
| Location: | 4520525 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451250 | (1 of 3) PTHR13620:SF0 - EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCCCAGCGCCCGCTTGTGCAGCTTCTGCCGCCGCCGCCGCTGCCCC |
| Internal bar code: | GTTAACTTAACTCACCTTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3393 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGCTGTGACATTGTTGT |
| Suggested primer 2: | TTGGACATGCTAGCCAGTCG |