| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029523 |
| Chromosome: | chromosome 6 |
| Location: | 3753986 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278165 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGCCAGCCAGGACTTTGCAAAGTCCCACAGCCCCCAGATCTCGGGG |
| Internal bar code: | GCCAAGGTGGTTTACCTAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2809 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTTTAGGGGCACAGTA |
| Suggested primer 2: | CATGCCAGGCTTGTTGTGAG |