| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029525 |
| Chromosome: | chromosome 4 |
| Location: | 2283229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGAGATTGACTCCTATGATTGCTCGGGTAGTTGGTGTGAGGAGTTG |
| Internal bar code: | GCAACAACAAGCGAGTTTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAAGGAATCGCTGGTGG |
| Suggested primer 2: | CGAAGGTTGTGTGGGGATGA |