Insertion junction: CLIP2.029540_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029540
Chromosome:chromosome 17
Location:716505
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g700700 (1 of 37) IPR008979 - Galactose-binding domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACAAGGTTGCTGGCCGCTTCCAAGTCTTCTTGAAGCAGCCACGCATCC
Internal bar code:ATTCTAGTACTGTGACTTTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1123
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GTGATCACCACGATGCATGC
Suggested primer 2:AACCATGATTCCGACACGCT