Insertion junction: CLIP2.029545_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029545
Chromosome:chromosome 13
Location:1117810
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569350 SNE16 (1 of 1) PTHR10366//PTHR10366:SF267 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // PROTEIN F13D11.4; NAD dependent epimerase/dehydratase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACAGCGTTAAAATGGCGGCGAGGCTGCATCCTGCTGGGATGCAGGCTG
Internal bar code:GACCAGACATAGCTACAGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3063
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGTTGAAGTACGGGGTT
Suggested primer 2:GGGTAGGTGTTACGTGGCAA