| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.029567 |
| Chromosome: | chromosome 13 |
| Location: | 2639207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581150 | NAT30 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCCCTTCCACACACTGCCGCACGCAGTCCCTCTTACCCACGCGCTC |
| Internal bar code: | CAATCGCTGGACAATGTGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3530 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCTGGAGCGTGTGTTGA |
| Suggested primer 2: | CCCCCACTTCCCTAGCTAGT |