Insertion junction: CLIP2.029573_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.029573
Chromosome:chromosome 2
Location:1819105
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g087000 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAAGCGGCTGTAAGCGGCTCAATATGCTCGTGTAGCAAGGGCGGCAC
Internal bar code:GTCTTGAAGATATGGGTGGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1250
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGGTACCACTGAACGTC
Suggested primer 2:CCCACTTTGCCTGCTCCATA