Insertion junction: CLIP2.029590_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029590
Chromosome:chromosome 12
Location:8816769
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g546800 PHC53 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCAGATACCTTGCTCACAGCAGGCAGGCACGTCGATGTACTTTGGC
Internal bar code:TTTCTCACGTATGTACTGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2121
LEAP-Seq percent confirming:84.2105
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCCCACTACCCCTTCCG
Suggested primer 2:TAGCTTTGCACGTGAGCTCA