| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.029590 |
| Chromosome: | chromosome 12 |
| Location: | 8816769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546800 | PHC53 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCAGATACCTTGCTCACAGCAGGCAGGCACGTCGATGTACTTTGGC |
| Internal bar code: | TTTCTCACGTATGTACTGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2121 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTCCCACTACCCCTTCCG |
| Suggested primer 2: | TAGCTTTGCACGTGAGCTCA |